Transporter Associated Protein (TAP1, Transporter Associated with Antigen Processing) (Not for Export EU)

Size

£672.00

SKU:: T8266-05
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to a portion of human TAP1.
Format:: Supplied as a liquid. No preservatives added.
Extra Details:: TAP1, a 70kDa transmembrane protein, and TAP2 are two structurally related subunits of the transporter associated with antigen processing (TAP). The TAP complex is a member of the ATP binding cassette (ABC) family of transmembrane transporters. TAP1 and TAP2 each contain an N-terminal transmembrane region and C-terminal nucleotide binding domains (NBD). TAP1 and TAP2 form a complex in the endoplasmic reticulum (ER) membrane with the NBD oriented in the cytosol (1). The TAP transporter is an essential component of the MHC class I antigen presentation pathway by binding peptides in its cytosolic part and subsequently translocating the peptides into the lumen of the endoplasmic reticulum (ER) where assembly of MHC class I and pepide takes place. Assembly of MHC class I-beta2-micorglobulin (beta2-m) dimers in the ER involves 2 chaperones, calnexin which interacts with free class I heavy (H) chains and calreticulin which binds human class I-beta2 dimers prior to peptide loading. Calreticulin remains associated with at least a subset of class I molecules when they in turn bind to TAP (2). Polymorphic differences in MHC class I H chains can results in quantitative as well as qualitative differences in how they interact with peptide, beta2-m, calnexin, calreticulin, ERp57, TAP and Tapasin, a subunit of the TAP complex which binds to both TAP1 and MHC class I (3,4). Data obtained with Tapasin deletion mutants revealed that binding to TAP is mediated by the C-terminal region and that the N-terminal region is required to stabilize the MHC class I loading complex (5). The Tapasin gene is centromeric of HLA-DP locus between the HSET and HKE1.5 genes and within 500 kbp of the transporters associated with antigen processing, TAP1 and TAP2 genes. The localization of these genes within such a short distance of each other on the chromosome implies some regulatory or functional significance (6). Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot (ECL): 1:1000-1:4000, detects a band of ~70kD Immunoprecipitation: 1:100 Immunohistochemistry: paraffin Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice