T8260-04
Transglutaminase II
Size
£971.00
- SKU:
- T8260-04
- Purity:
- Purified by Protein G affinity chromatography from ascites.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified guinea pig liver TGase II. Cellular Localization: Cytoplasm, cell membrane, and extracellular matrix.
- Clone:
- 3F382
- Format:
- Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
- Extra Details:
- Transglutaminase II catalyzes calcium-dependent post-translational modification of proteins by formation of an isopeptide bond within or between polypeptide chains. It is also known as TGC, tTG, type II-, Gh, cytosolic-, liver-, endothelial-, erythrocyte-, cellular-transglutaminase. Different tissues and cell types express varying amounts of tissue transglutaminase with a markedly hugh expression in rheumatoid lesions. It is implicated in programmed cell death, signal transduction, drug resistance, cell growth, endocytosis, insulin secretion, cell adhesion, cataract formation, and wound healing. Applications: Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot, Immunohistochemistry, Affinity Purification and Biological Blockade. Other applications have not been tested. Recommended Dilution: Affinity Purification: Use without BSA Biological Blockade: Use without azide Immunoprecipitation (Use Protein G): 2ug/mg protein lysate. Western Blot: 1-2ug/ml for 2hrs at RT Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions to be determined by the researcher. Positive Control: HUVEC cells. Breast carcinoma. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice