T8250-19C
TGF-beta 1, 2, 3
Size
£889.00
- SKU:
- T8250-19C
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant. Endotoxin: ≤0.1EU/ug (LAL).
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Bovine bone-derived TGF-beta 1 and TGF-beta 2
- Clone:
- 10F520 (1D11)
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Transforming growth factor-beta (TGF-beta) family of proteins has potent hematopoietic regulatory properties ranging from effects on the growth and differentiation of primitive stem cells to the differentiated functions of mature cells. Applications: Suitable for use in ELISA, Neutralization and Western Blot. Other applications not tested. Recommended Dilutions: Neutralization: Measured by its ability to neutralize TGF‑ beta 1 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line. Tsang, M. et al. (1990) Lymphokine Res. 9:607. The Neutralization Dose (ND50) is typically 0.25-1.25ug/ml in the presence of 1ng/mL recombinant human TGF‑ beta 1 and 7.5 ng/ml recombinant mouse IL‑4. Western Blot: 1ug/ml under non-reducing conditions only. ELISA (capture): 2-8ug/ml using T8250-16B as detection antibody. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice