T8195-02

Transcription Elongation Regulator 1 (TCERG1, CA150, MGC133200, TAF2S, TATA box-binding protein-associated factor 2S, Transcription factor CA150, Urn1)

Size

£448.00

SKU:: T8195-02
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant corresponding to a partial sequence of human Transcription Elongation Regulator 1.
Clone:: 8C333
Format:: Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Extra Details:: This gene encodes a nuclear protein that regulates transcriptional elongation and pre-mRNA splicing. The encoded protein interacts with the hyperphosphorylated C-terminal domain of RNA polymerase II via multiple FF domains, and with the pre-mRNA splicing factor SF1 via a WW domain. Applications: Suitable for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilutions: Immunofluorescence (IC): 2-100ug/ml. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. Western Blot: 0.2-2ug/ml Flow Cytometry: 0.5-2ug/ml. HeLa cells with 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.