T8180

TRAIL, Recombinant, Human (TNF-Related Apoptosis Inducing Ligand, Apo2L, TL2, TNFSF10)

Size

£364.00

SKU:: T8180
Molecular Weight:: 19.6
Purity:: ≥98% (SDS-PAGE and HPLC). Endotoxin: ≤0.1ng/ug (1EU/µg)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from PBS. No preservative added. Reconstitute with sterile PBS to a concentration of ≥0.1-1mg/ml.
Extra Details:: Human TRAIL (TNF-Related Apoptosis Inducing Ligand, Apo2 Ligand), a 19.6kD protein consisting of 169 amino acid residues, is a cytotoxic protein which activates rapid apoptosis in tumor cells, but not in normal cells. Biological Activity: Assay #1: Determined by its ability to induce apoptotic cell death in TRAIL-sensitive U343MG cells. The expected ED50 for this effect is 1-3ng/ml. Assay #2: Measured by its ability to induce apoptosis in LN-18 cells (human glioblastoma cells). The expected ED50 for this effect is 0.8-2ng/ml. Amino Acid Sequence: MRERGPQRVA AHITGTRGRS NTLSSPNSKN EKALGRKINS WESSRSGHSF LSNLHLRNGE LVIHEKGFYY IYSQTYFRFQ EEIKENTKND KQMVQYIYKY TSYPDPILLM KSARNSCWSK DAEYGLYSIY QGGIFELKEN DRIFVSVTNE HLIDMDHEAS FFGAFLVG Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.