T8067-55B
Torc1 (Transducer of Regulated CREB Activity 1)
Size
£787.00
- SKU:
- T8067-55B
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Gln384-Gln471 from mouse TORC1, expressed in E. coli. Species sequence homology: rat (97%), human (90%).
- Clone:
- 9L860
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- TORC1, also known as CREB-regulated transcription coactivator 1 (crtc1), is a 67kD member of the TORC family. Mouse TORC1 is 630aa in length. It contains multiple potential sites for phosphorylation and a serine-rich region from aa295-365. Mouse TORC1 is 97% and 90% aa identical to rat and human TORC1, respectively. TORC1 is highly expressed in specific regions of the brain including the cortex, hippocampus, and striatum. It functions as a transcription coactivator for CREB1, which activates transcription through both consensus and variant cAMP response element (CRE) sites. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 8-25ug/ml with perfusion fixed frozen sections of mouse brain (hippocampus). Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice