T8067-50C-HRP
Toso, CT (Regulator of Fas-induced Apoptosis, Fas Apoptotic Inhibitory Molecule 3, FAIM3) (HRP)
Size
£947.00
- SKU:
- T8067-50C-HRP
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- A 13aa peptide from near the carboxy terminus of human Toso.
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
- Extra Details:
- Toso, also known as Fas Apoptotic Inhibitory Molecule 3 (FAIM3). Toso is expressed by lymphoid and hematopoietic cells and is up-regulated by T-cell activation. Toso expressing cells have increased resistance to Fas-, FADD-, and TNFalpha-induced apoptosis. Toso is membrane bound, and is thought to directly bind to FADD, disrupting DISC formation and thus inhibiting caspase-8 activation. Toso is a potential proto-oncogene, and could be a therapeutic target for use in the treatment of autoimmune diseases. Despite its predicted molecular weight, Toso often migrates at 60kD in SDS PAGE. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml detects a band of ~43kD in human lung tissue lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice