T8065-11
Topoisomerase II (DNA) alpha
Size
£793.00
- SKU:
- T8065-11
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Nuclear protein preparation from the human cell line U937.50
- Clone:
- 2Q382
- Format:
- Supplied as a liquid in PBS, pH 7.6, 0.09% sodium azide.
- Extra Details:
- The expression of topoisomerase II alpha is strongly restricted to proliferating cells. The topoisomerase II alpha antigen is preferentially expressed during G1, S, G2 and M phase of the cell cycle, while resting, non-cycling cells (G0 phase) lack topoisomerase II alpha. In addition, constantly proliferating cells (e.g. cell lines) react positively to topoisomerase II alpha during the entire cell-cycle. This specificity for proliferating cells might make it a useful tool for determination of the proliferative fraction in solid tumors such as mammary carcinomas and gangliomas. Applications: Suitable for use in Immunohistochemistry, Western Blot, Immunoprecipitation, Immunocytochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 1-10ug/ml Immunohistochemistry: 5-10ug/ml. Strong nuclear staining only in proliferating cells. The epitope recognized by the antibody is also detectable in paraffin-embedded tissue sections. Immunocytochemistry: 5-10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice