T8050-84

TLR8 (Toll Like Receptor 8, Toll-like Receptor 8, TLR 8, CD288, CD288 Antigen, MGC119599, MGC119600)

Size

£659.00

SKU:: T8050-84
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: KLH-conjugated synthetic peptide corresponding to human TLR8, within amino acids 750-850.
Clone:: 4H283 (44C143)
Format:: Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Extra Details:: The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TLR8 gene contains three exons, two of which have coding function (4). TLR8 cDNA codes for a protein of approximate molecular weight of 120kD (4,5). Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Western Blot, Immunocytochemistry and Immunofluorescence. Other applications have not been tested. Recommended Dilutions: Western Blot: 1-3ug/ml. Cell lysate from 293 transfected with human TLR8 was used as positive control. A protein of~20kD was detected. Flow Cytometry (intracellular): 2-5ug/1x10e6 cells. Ramos cells can be used as a positive control. Immunohistochemistry (paraffin): 5ug/ml. Requires HIER using sodium citrate buffer pH6.0 prior to staining. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice