TLR7 (Toll Like Receptor 7, TLR-7, PRO285, UNQ248)

Size

£665.00

SKU:: T8050-73
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa706-728 of human TLR7 (KLH coupled).
Format:: Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Extra Details:: The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1) and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described (3). Stimulation of the NFkB signaling pathway by TLR7 suggests that it plays a role in immune response. Applications: Suitable for use in Immunohistochemistry, Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 2-5ug/1x10e6 (Intracellular staining). Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Ramos or Raw cells Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice