Toll Like Receptor 7 (Toll-like Receptor 7, TLR7, HGNC:15631, UNQ248/PRO285) (FITC)

Size

£806.00

SKU:: T8050-72Q
Application:: Flow Cytometry
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa706-728 of human TLR7 (KLH).
Format:: Supplied as a liquid in 10mM Tris, 150mM sodium chloride, 0.05% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Extra Details:: The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1) and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described (3). Stimulation of the NFkB signaling pathway by TLR7 suggests that it plays a role in immune response. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow (Cell Surface): 2-5ug/ 1x10e6 cells, see Patole et al (2006) and Pawar et al (2006) for details Flow (Intracellular): 0.5-1ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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