T8050-53L

TLR6 (Toll-like Receptor 6, CD286) (PE)

Size

£691.00

SKU:: T8050-53L
Application:: Flow Cytometry
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to aa408-424 of human TRL6.
Clone:: 86B1153.2
Format:: Supplied as a liquid in PBS, 0.05% sodium azide. Labeled with R-Phycoerythrin (PE).
Extra Details:: The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). TLRs characterized so far activate the MyD88:interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described. the aa sequence of human TLR6 is most similar to hTLR1 with 69% identity at the aa level. Human TLR6 consists of a predicted 807aa with a molecular weight of approximately 92kD (3). Human and mouse TLR6 share an aa identity of 73%. TLR6 activates both NF-kB and c-Jun N-terminal Kinase (JNK). the aa in the cytoplasmic domain of IL-1 receptor, which are critical for NF-kB activation, are conserved in TLR6. It has also been shown that TLR2-mediated response to a phenol-soluble factor from staphylococcus epidermidis is enhanced by TLR6 (4). TLR6 is predominantly expressed in spleen, thymus, ovary, and lung (3). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry (Intracellular): 0.5-1ug/10e6 cells Optimal dilutions to be determined by the researcher. Positive Control: Ramos, human PBMCs Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

Shipping Conditions:: Blue Ice