T8050-34D

TLR4 (Toll Like Receptor 4, CD284) (PE)

Size

£320.00

SKU:: T8050-34D
Application:: Flow Cytometry
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Ba/F3 cell line expressing TLR4.
Clone:: 3F5 (HTA125)
Format:: Supplied as a liquid in PBS, 1% BSA, 5% sucrose, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Extra Details:: TLR4 has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 mediates the innate immune response to bacterial lipopolysaccharide. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: Neat. Use 10ul to label 10e6 cells or 100ul whole blood. Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells from immunized Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.