T8050-34C
TLR4 (Toll Like Receptor 4, CD284) (FITC)
Size
£422.00
- SKU:
- T8050-34C
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Ba/F3 cell line expressing TLR4
- Clone:
- 3F5 (HTA125)
- Format:
- Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- TLR4 has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 mediates the innate immune response to bacterial lipopolysaccharide. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat. Use 10ul to label 10e6 cells or 100ul whole blood. Optimal dilutions to be determined by the researcher. Recommended Negative Control: I1904-83Y1: IgG2a Negative Control (FITC) Hybridoma: SP2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice