T5585-10A
Tissue Inhibitor of Metalloproteinases 2 (TIMP2)
Size
£978.00
- SKU:
- T5585-10A
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide from the N-terminal region of human TIMP-2 protein. Cellular Localization: Cytoplasmic MW of Antigen: 21kD Epitope: N-terminal
- Clone:
- 3F373
- Format:
- Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide
- Extra Details:
- TIMP-1 and TIMP-2 are inhibitory enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-2 shows the highest binding affinity to both the latent (pro) and active forms of 72kD Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92kD Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. Applications: Suitable for use in Western Blot and Immunohistochemistry. Not suitable for Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml for 2hrs at RT. Immunohistochemistry (Formalin/paraffin only): 2-4ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilution determined by the researcher. Positive Control: Conditioned, serum-free medium from (TPA-treated) HFL-1 cells. Pancreatic or colon carcinoma. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice