TIM-1 (T Cell Immunoglobin Domain and Mucin Domain Protein 1, TIM 1, TIM1, TIMD 1, TIMD1, TIMD-1, Hepatitis A Virus Cellular Receptor 1, HAVCR 1, HAVCR1, HAVCR-1, Kidney Injury Molecule 1, KIM1, KIM-1) [KO Validated]

Size

£655.00

SKU:: T5469-91D
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Peptide corresponding to 16aa from near the center of human TIM-1. Epitope: aa230-280. Species Sequence Homology: mouse (50%) and rat (43.7%)
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: TIM-1 was first identified as the receptor used by hepatitis A for viral entry (it is also known as the hepatitis A virus cellular receptor- HAVCR). TIM-1 is a key cell surface protein that binds TIM-4, leading to T cell proliferation and T helper type 2 (Th2) cytokine production. TIM-1 has also been shown to bind and activate macrophages. Two splice variants for human TIM-1 exist; TIM-1a is predominantly expressed in the liver, whilst TIM-1b is expressed in the kidney. Expression of TIM-1 in the kidney is thought to be upregulated in damaged tissue, and is thought to assist healing. TIM-1 polymorphisms are associated with asthma. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1-8ug/ml detects a band of ~49kD in human uterus tissue lysate. siRNA Knockdown Validation: T5469-91D specificity was further verified by TIM-1 specific siRNA knockdown. TIM-1 signal in HeLa cells transfected with TIM-1 siRNAs was much weaker in comparison with that in HeLa cells transfected with control siRNAs. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice