THRB (Thyroid Hormone Receptor beta, Nuclear Receptor Subfamily 1 Group A Member 2, c-erbA-2, c-erbA-beta, ERBA2, NR1A2, THR1)

Size

£642.00

SKU:: T5349-01K
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-REELQKSIGHKP, from human THRB, at the internal region of the protein (NP_000452.2).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Thyroid hormone receptors (THRs) are 55kD ligand dependent members of the steroid/retinoic acid superfamily of transcription factors. The two genes encoding THRs identified to date, TRa and TRb, have been mapped to human chromosomes 17 and 3, respectively. THRs bind to thyroid hormone response elements (THREs) with half site binding motifs in the orientation of palindromes, direct repeats, or inverted palindromes. The affinities of binding are both variable and influenced differentially by 3,5,3'-triiodo- L-thyronine (T-3). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Western Blot: 0.3-1ug/ml, observed in human bone marrow lysates on 50+60kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.