T5300-17F
Thyroglobulin (Tg, Tgn, AITD 3, AITD3, hTG)
Size
£544.00
- SKU:
- T5300-17F
- Application:
- IHC-Fr
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Thyroid follicular cells
- Clone:
- 2H11
- Format:
- Supplied as liquid in PBS, pH 7.4, 15mM sodium azide.
- Extra Details:
- Thyroglobulin is the glycoprotein precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). Thyroglobulin is obtained from the thyroid gland and exhibits the general properties of the globulins. The human thyroglobulin (hTG) is a high molecular weight glycoprotein (605kD) found in the thyroid follicular cells. It plays a central role in the uptake, incorporation, and regulated biosynthesis of thyroid hormones, T4 and T3. Thyroid disorders are, in large part, due to autoimmune origin, and anti thyroglobulin autoantibodies were the first factor to be discovered. Anti hTG is found in all thyroid autoimmune diseases (Hashimoto’s thyroiditis, Graves’s diseases), with the highest level observed in Hashimoto’s thyroiditis. Anti hTG is also characteristic of thyroid cancer, and its determination can be used for the follow up of cancer patients. TG is mainly expressed on thyroid follicular cells (99.1%). Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (paraffin sections): 1:80 -1:100; pretreatment: heat retrieval in 0.01M citrate buffer, pH 6.o (microwave oven). Positive tissue: thyroid gland, follicular adenoma. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice