T5155-10D
Thymidylate Synthase (Thymidylate Synthetase, TMS, TS, TSase, TYMS, TYMS Protein, Tyms Thymidylate Synthetase, dTMP Synthase, EC 2.1.1.45, HsT422, MGC88736) (Azide Free)
Size
£685.00
- SKU:
- T5155-10D
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Partial recombinant protein corresponding to a portion of amino acids within aa111-211 from human Thymidylate synthase, GST tag. MW of the GST tag alone is 26kD (P04818).
- Clone:
- 10B2490
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free).
- Extra Details:
- Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase. Applications: Suitable for use in Immunofluorescence (IC) and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice