T5152-11
TDRKH, Tudor domain and KH domain containing protein, TDRD2
Size
£806.00
- SKU:
- T5152-11
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Recombinant corresponding to His206-Leu561 form human TDRKH expressed in E. coli.
- Format:
- Supplied as a lyophilized powder from PBS with 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- TDRKH (Tudor domain and KH domain containing protein; also TDRD2) is a 70kD member of the Tudor family of proteins. It is expressed in developing spermatogonia and meiotic primary spermatocytes, but not embryonic stem cells. TDRKH forms part of an RNA-silencing complex that safeguards the germ cell genome. Through its 60aa Tudor domain, it binds methylated PIWI (P-element-induced wimpy testis) protein and serves to position PIWI for non-coding RNA binding. Human TDRKH is 606aa in length. It contains two RNA recognition KH domains (aa52-115 )and (124-190), and one methyl-binding Tudor domain (aa353-412). There are four potential splice forms. Three possess a shared deletion of (aa562-606), with two of these also showing a unique deletion of (aa108-152) and 76-79, respectively; a fourth shows a two aa substitution for (aa559-606). Over (aa206-561), human TDRKH shares 87% aa identity with mouse TDRKH. Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry: 5-15ug/ml Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice