Thrombospondin (TSP) (Not for Export EU)

Size

£704.00

SKU:: T5100-08A
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Thrombospondin, a glycoprotein, has been purified from platelet lysate.
Format:: Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with 1ml sterile dH2O.
Extra Details:: Thrombospondin is a protein from platelet a granules. It is secreted at sites of platelet activation and aggregation and is involved in the differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells. Applications: Suitable for use in ELISA, Double Radial Immunodiffusion and Immunoelectrophoresis. Other applications not tested. Recommended Dilution: Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10ul in 3mm diameter center well and 2ul serum samples (neat and serially diluted in 2mm diameter peripheral wells). Immunoelectrophoresis: 120ul against 2ul serum or equivalent Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.