T5100-06DX
Thrombospondin (TSP) (BSA & Azide Free)
Size
£978.00
- SKU:
- T5100-06DX
- Purity:
- Purified by Protein G affinity chromatography from ascites.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets. MW of Antigen: ~450kD (non-reduced), 170-180kD (reduced). Epitope: Collagen Type V-binding domain of TSP.
- Clone:
- 3F353
- Format:
- Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See T5100-06D.
- Extra Details:
- Thrombospondin is a protein from platelet a-granules. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells. Applications: Suitable for use in Electron Microscopy, Flow Cytometry. Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunoprecipitation (Native verified): 2ug/mg protein lysate, Use Protein G. Immunohistochemistry (Formalin/paraffin): 1:25-1:50 for 20 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Western Blot: 1-2ug/ml for 2 hours at RT. Optimal dilution determined by the researcher. Positive Control: Platelets in placenta, megakaryocytes in bone marrow Cellular Localization: Secretory granules, Golgi complex, endoplasmic reticulum, extracellular matrix Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice