T5100-06DX-PE
Thrombospondin (TSP) (BSA & Azide Free) (PE)
Size
£1,234.00
- SKU:
- T5100-06DX-PE
- Purity:
- Purified by Protein G affinity chromatography from ascites.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets. MW of Antigen: ~450kD (non-reduced), 170-180kD (reduced). Epitope: Collagen Type V-binding domain of TSP.
- Clone:
- 3F353
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- Thrombospondin is a protein from platelet a-granules. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells. Applications: Suitable for use in Electron Microscopy, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Formalin/paraffin): Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution determined by the researcher. Positive Control: Platelets in placenta, megakaryocytes in bone marrow Cellular Localization: Secretory granules, Golgi complex, endoplasmic reticulum, extracellular matrix Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice