T5100-06DX-BIOTIN

Thrombospondin (TSP) (BSA & Azide Free) (Biotin)

Size

£1,234.00

SKU:: T5100-06DX-BIOTIN
Purity:: Purified by Protein G affinity chromatography from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Reduced and alkylated puriﬁed human TSP (fully denatured) from the supernatant of thrombin-activated platelets. MW of Antigen: ~450kD (non-reduced), 170-180kD (reduced). Epitope: Collagen Type V-binding domain of TSP.
Clone:: 3F353
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Extra Details:: Thrombospondin is a protein from platelet a-granules. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells. Applications: Suitable for use in Electron Microscopy, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Formalin/paraffin): Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution determined by the researcher. Positive Control: Platelets in placenta, megakaryocytes in bone marrow Cellular Localization: Secretory granules, Golgi complex, endoplasmic reticulum, extracellular matrix Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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