T5100-06D
Thrombospondin (TSP)
Size
£971.00
- SKU:
- T5100-06D
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets
- Clone:
- 3F353
- Format:
- Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See T5100-06DX
- Extra Details:
- Thrombospondin is a protein from platelet a-granules. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells. Applications: Suitable for use in Electron Microscopy, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Immunoprecipitation (Native verified) (Use Protein G): 2ug/mg protein lysate Western Blot: 1-2ug/ml for 2hrs at RT Immunohistochemistry (Formalin/paraffin): 1:25 to 1:50 for 60 min at RT Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min Optimal dilutions to be determined by the researcher. MW of Antigen: ~450kD (non-reduced); 170 to 180kD (reduced) Epitope: Collagen Type V-binding domain of TSP Positive Control: Platelets in placenta, megakaryocytes in bone marrow. Cellular Localization: Secretory granules, Golgi complex, endoplasmic reticulum, extracellular matrix Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice