T5050-06E

Thrombopoietin (TPO, Mpl-ligand, MGDF)

Size

£774.00

SKU:: T5050-06E
Purity:: Purified from ascites
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant human TPO
Clone:: 7C200
Format:: Supplied as a liquid in PBS, 10mg/ml BSA
Extra Details:: TPO (Thrombopoietin, Mpl-ligand, MGDF) stimulates the proliferation and maturation of megakaryocytes and promotes increased circulating levels of platelets in vivo. Human TPO is a soluble 18.5kD protein containing 174 amino acid residues which comprises the receptor binding domain of the Mpl-ligand protein. Applications: Suitable for use in Indirect ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.2ug/ml detects a single band at ~46kD. Indirect ELISA: 50ng will yeild an OD of >2.0 under standard assay conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.