T5048-04

Thrombomodulin (CD141, TM, Fetomodulin)

Size

£825.00

SKU:: T5048-04
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to Leu17-Ser517 from mouse Thrombomodulin expressed in NSO cells.
Clone:: 8H203
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Thrombomodulin is a 110-120kD, endothelial cell-specific type I transmembrane glycoprotein. It binds thrombin, rendering it unavailable for clotting, and activates protein C, which also serves as an anticoagulant. Mouse Thrombomodulin is 577aa in length. It contains a 510aa extracellular domain (ECD) (aa17-517) that shows one C-type lectin domain (aa31-167) and six EGF-like regions (aa240-480). Thrombin binds to aa403-480. One isoform may exist that shows a deletion of the C-terminal 86 amino acids (aa492-577). Over aa17-517, mouse Thrombomodulin is 66%, 85% and 67% aa identical to human, rat and canine TM, respectively. Applications: Suitable for use in Flow Cytometry, Direct ELISA, Western Blot Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug/ml; 10ul labels 1x10e6 bEnd.3 cells Western Blot: 1ug/ml. Using a colorimetric detection system, the detection limit for recombinant mouse Thrombomodulin is ~25ng/lane under non-reducing conditions. Use under reducing conditions is not recommended. Immunohistochemistry: 8-25ug/ml detects Thrombomodulin in frozen sections of normal mouse kidney and heart. Immunocytochemistry: 8-25ug/ml. Used with bEnd.3 cells. Cells were fixed with PBS, 4% paraformaldehyde and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA. Between each step, cells were washed with PBS, BSA. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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