T5045

Thrombin

Size

£442.00

SKU:: T5045
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified human Thrombin
Clone:: 8.H.10
Format:: Supplied as a liquid containing 50% glycerol/50% water (vol/vol).
Extra Details:: Thrombin is a key clot promoter. It is an enzyme that presides over the conversion of a fibrinogen to fibrin. The coagulation cascade, is a series of proenzyme-enzyme conversions. Coagulation starts when a vessel is wounded. A vessel is wrapped in a layer that is full of cells containing Tissue Factor (TF). After injury, TF is exposed to blood, it binds with factor VIIa to form the first enzyme: TF:VIIa. This activates factor X to Xa, which in turn forms thrombin. Applications: Suitable for use in ELISA. Not suitable for Western Blot. Reported to be suitable for IHC. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Extinction Coefficient (1cm, 280nm): 14.0 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.