T5027
Threonine-X-Arginine (Thr-X-Arg), phosphorylated
Size
£704.00
- SKU:
- T5027
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide containing the phospho-Thr-X-Arg motif.
- Format:
- Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
- Extra Details:
- Some important signaling proteins can be regulated by phosphorylation at a specific threonine followed by arginine or lysine at the +2 position. For example, conventional PKC isozymes phosphorylate substrates containing serine or threonine with Arg or Lys at the-3,-2 and +2 positions (1–2). c-Raf, a mitogen-activated protein kinase and the main effector recruited by GTP-bound Ras, is phosphorylated at Thr481 and Thr491 followed by Lys at the +2 position (3). APP1 and ERF also contain a phosphorylated Thr followed by Arg or Lys at the +2 position. Phosphorylation of these sites is important for their activities. In order to determine the phosphorylation state of Thr in the Thr-X-Arg motif and to identify potential new phosphorylation sites in this motif, antibodies have been developed that recognize phosphorylated Thr followed by Arg or Lys at the +2 position. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:800 Requires HIER using sodium citrate buffer pH6.0 prior to staining. ELISA Peptide: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice