T5026
Threonine-Proline (Thr-Pro), phosphorylated
Size
£639.00
- SKU:
- T5026
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic phosphothreonine-proline-containing peptides. Antibody is a mouse IgM clone and can be recognized by anti-mouse Ig (whole molecule) secondary antibody.
- Clone:
- 3A40(P-Thr-Pro-101)
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
- Extra Details:
- The MAPK and CDK family of serine/ threonine protein kinases plays important roles in cell signaling and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (1–6). To study and discover new MAPK and CDK substrates. As determined by ELISA using a wide variety of phospho-Thr-Pro peptides, Phospho-Threonine-Proline Monoclonal Antibody (P-Thr-Pro-101) recognizes the phospho-Thr-Pro motif in a highly context-independent fashion. It also interacts with a broad range of phospho- Thr-Pro-containing proteins as determined by Western analysis of 2D gels of nocodazole treated Jurkat cell extracts. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:5000. Incubate membranes with diluted antibody, 1X TBS, 5% BSA, 0.1% Tween-20 at 4ºC with gentle shaking overnight Immunohistochemistry (Paraffin): 1:400 ELISA (Peptide): 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice