T5010-10E

Thioredoxin-1, aa2-109, Recombinant, E. coli (Trx-1, trxA, b3781, dasC, fipA, JW5856, tsnC)

Size

£607.00

SKU:: T5010-10E
Application:: WB
Molecular Weight:: 12.8
Purity:: ≥95% (SDS-PAGE). Purified by using conventional chromatography techniques.
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris HCl, pH 8.0, 1mM DTT, 10% glycerol.
Extra Details:: Thioredoxin 1 (Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. Recombinant enzyme corresponding to aa2-109 of Thioredoxin-1 fused to His-tag at N-terminus, was expressed in E. coli. Amino Acid Sequence: MHHHHHHMGS DKIIHLTDDS FDTDVLKADG AILVDFWAEW CGPCKMIAPI LDEIADEYQG KLTVAKLNID QNPGTAPKYG IRGIPTLLLF KNGEVAATKV GALSKGQLKE FLDANLAGS Specific Acitivity: >70 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650nm resulting from the reducution of insulin. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.