T2400-02F
Telomeric Repeat Binding Factor 2 (TRF2, TERF2, telomeric DNA-binding protein)
Size
£837.00
- SKU:
- T2400-02F
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant human TRF2 aa78-238; Q15554 (E. coli).
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- TRF-2 (telomeric repeat-binding factor 2; also telomeric DNA-binding protein) is a 55-60kD, ubiquitously expressed nuclear protein that participates in telomere homeostasis. TRF-2 binds as a dimer to TTAGGG repeats at ends of chromosomes (telomeres), where it blocks inappropriate activation of the ATM/p53 pathway. It also collaborates with TRF-1 to promote normal telomere length. TRF2 has been found up regulated in several human cancers. Human TRF-2 is 500 aa in length. It contains an N-terminal Arg-rich region (aa13-30), a dimerization domain (aa46-112), an NLS (aa329-333), and a DNA binding HTH myb-type domain (aa442-499). There is one potential alternate start site 42aa upstream of the standard start site, and one splice form that shows a 13aa substitution for aa239-500. Over aa78-238, human TRF-2 shares 96% aa identity with mouse TRF-2. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2.0ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice