T2040-25D

TCR, V beta 8.1, 8.2 (T Cell Receptor Vb8.1, 8.2 Chain, T Cell Receptor)

Size

£326.00

SKU:: T2040-25D
Application:: Flow Cytometry
Purity:: Purified by affinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: TCR receptor from T cell hybridoma DO-11.10
Clone:: KJ16-133.18
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Extra Details:: The TCR Vb 8 family consists of three members, namely Vb8.1, 8.2 and 8.3. This antibody reacts with Vb8.1, 8.2 only, but not Vb8.3. Most mouse strains have about 20% Vb 8+ T cells in the periphery. However, C57BR, C57L, SJL, SWR (Tcra haplotype) and RIII strains (Tcrc haplotype) do not express Vb8 due to a gene deletion at the Vb 8 family loci. Other strains expressing endogenous super-antigen, such as Mls-1a have significant reduced number of Vb 8.1 cells in the periphery. Exogenous superantigen, such as staphylococcal enterotoxin B, stimulates lymphocytes bearing Vb 8 and selectively eliminate those T cells in vivo. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: ≤0.25ug/10e6 cells in 100ul volume Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.