T2040-17D
TCR, Vbeta3 (T Cell Receptor V-beta-3, TCR V-beta-3)
Size
£576.00
- SKU:
- T2040-17D
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human HA1.7 TCR chain expressed on transgenic mouse cells
- Clone:
- 5C95
- Format:
- Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride. No preservative added.
- Extra Details:
- Human T cell receptor (TCR) is a T cell surface expressed heterodimer of alpha/beta or gamma/delta chains. TCRs recognize antigen peptides bound to MHC molecules forming the basis of antigen specific response by T cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5ug/ml; 80ul labels 5x10e5 cultured CH7C17 tumor cells. Optimal dilutions to be determined by the researcher Flow Cytometry Protocol: 1. Five x 10e5 cultured CH7C17 tumor cells were washed and incubated 45 minutes on ice with 80ul of T2040-17D at 5ug/ml. 2. Cells were washed twice and incubated withI 1904-04E after which they were washed three times, fixed and analyzed by FACS. 3 Cells stained positive with a mean shift of 0.9 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice