T2040-06C

TCR, gamma, delta (T Cell Receptor) (PE)

Size

£422.00

SKU:: T2040-06C
Purity:: Purified
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Clone:: TCR1
Format:: Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE.
Extra Details:: This antibody precipitates a heterodimer of Mr 90kD (two bands of Mr 40kD and 50kD upon reduction) on chicken peripheral blood T cells.1,2 Deglycosylation of the heterodimer yields two polypeptides of Mr 35kD and 32kD from antibody precipitates. Unlike human and mouse, in which gamma/delta cells comprise a minor subset of T lymphocytes in the circulation, the chicken has a relatively large subset of gd T cells.1-3 The frequency of TCRgd cells is usually 20-25% of the total blood T cells, but may reach approximately 50% in chickens of 6 months of age.2 The majority of TCRgd+ cells in the thymus and blood are CD4-CD8-, although a small subset of them may express CD8 or CD4 coreceptors.3 However, when the TCRgd+ cells migrate into the spleen and intestine, most of them begin to express CD8. Although the biological function of gd T cells is unclear, they are clearly capable of cytotoxic activity in vitro. The CD8+ gd T cells may also be involved in down-regulation of the immune response.4 They cannot, however, induce a graft-vs-host (GVH) reactions.5 Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Immunohistochemistry: Frozen sections (acetone fixed). Flow Cytometry: ≤ 0.2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Shipping Conditions:: Blue Ice