T2040-06A
TCR, gamma, delta (T Cell Receptor) (FITC)
Size
£552.00
- SKU:
- T2040-06A
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Outbred chicken thymocytes and Ig-negative blood lymphocytes.
- Clone:
- 5K81
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with FITC.
- Extra Details:
- This antibody precipitates a heterodimer of Mr 90kD (two bands of Mr 40kD and 50kD upon reduction) on chicken peripheral blood T cells.1,2 Deglycosylation of the heterodimer yields two polypeptides of Mr 35kD and 32kD from antibody precipitates. Unlike human and mouse, in which gamma/delta cells comprise a minor subset of T lymphocytes in the circulation, the chicken has a relatively large subset of gd T cells.1-3 The frequency of TCRgd cells is usually 20-25% of the total blood T cells, but may reach approximately 50% in chickens of 6 months of age.2 The majority of TCRgd+ cells in the thymus and blood are CD4-CD8-, although a small subset of them may express CD8 or CD4 coreceptors.3 However, when the TCRgd+ cells migrate into the spleen and intestine, most of them begin to express CD8. Although the biological function of gd T cells is unclear, they are clearly capable of cytotoxic activity in vitro. The CD8+ gd T cells may also be involved in down-regulation of the immune response.4 They cannot, however, induce a graft-vs-host (GVH) reactions.5 Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation, and In ovo depletion of gd T cells. Other applications not tested. Recommended Dilution: Flow Cytometry: ≤1 ug/10^6 cells Immunohistochemistry : Acetone-fixed, frozen sections Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice