T2040-02W3

TCR, alpha, beta, V beta 2 (T Cell Receptor) (PE)

Size

£422.00

SKU:: T2040-02W3
Purity:: Purified
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Clone:: TCR3
Format:: Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE.
Extra Details:: This antibody precipitates a CD3-associated heterodimer of Mr 88kD (two bands of Mr 48kD and 40kD upon reduction) on chicken peripheral blood T cells.1,2 Deglycosylation of the heterodimer yields two polypeptides of Mr 34kD and 31kD from antibody precipitates. In the chicken, two distinct subpopulations of alpha/beta T cells appear in the thymus subsequent to the appearance of gamma/delta T cells. These subpopulations, originally denoted as TCR2 and TCR3,1,2 arise sequentially in the thymus during ontogeny and are now known to represent two distinct Vbeta families, Vb1 and Vb2, respectively.3 Two-color immunofluorescence has revealed that the TCR3+ thymocytes include CD4+CD8-, CD4-CD8+, CD4+CD8+ and CD4-CD8- subpopulations. The TCR3+ thymocytes can be separated into two subsets. One subset is characterized by relatively low levels of expression of the TCR3/CD3 complex and most of the cells in this subset are CD4+CD8+. Cells in the other subset express TCR3/CD3 in higher density and are either CD4+CD8- or CD4-CD8+, corresponding to the more mature medullary subset of thymocytes. The TCR3+ cells in the blood and spleen express relatively high levels of the TCR3/CD3 receptor complex and are “single positive, with CD4+CD8- cells being four times more frequent that the CD4-CD8+ cells (ca. 80% CD4+ vs ca. 20% CD8+).1,2 Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Immunohistochemistry: Frozen sections (acetone fixed). Flow Cytometry: ≤ 0.2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Shipping Conditions:: Blue Ice