T2040-02V3

TCR, alpha, beta, V beta 1 (T Cell Receptor) (PE)

Size

£422.00

SKU:: T2040-02V3
Purity:: Purified
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: White Leghorn chick thymocytes and blood mononuclear cells.
Clone:: TCR2
Format:: Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE.
Extra Details:: This antibody precipitates a CD3-associated heterodimer of Mr 90kD (two bands of Mr 40kD and 50kD upon reduction) on chicken peripheral blood T cells.1 Deglycosylation of the heterodimer yields two polypeptides of Mr 34kD and 29kD from antibody precipitates. In the chicken, two distinct subpopulations of alpha/beta T cells appear in the thymus subsequent to the appearance of gamma/delta T cells. These subpopulations, originally denoted as TCR2 and TCR3,1-3 arise sequentially in the thymus during ontogeny and are now known to represent two distinct Vb families, Vb1 and Vb2, respectively.4 Two-color immunofluorescence has revealed that most of the TCR2+ thymocytes express both CD4 and CD8 antigens. The TCR2+ cells in blood were found to express either CD4 (74±2%) or CD8 (26±4%). TCR2+ cells in the spleen also express either CD4 (37±1%) or CD8 (64±4%). Surprisingly, a relatively large subpopulation of CD8+ cells in the spleen are negative for TCR2.1-3 This observation led to the demonstration that 71±6% of the TCR1(gamma/delta)+ cells in the spleen express the CD8 marker.1-4 Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Immunohistochemistry: Frozen sections (acetone fixed). Flow Cytometry: ≤ 0.2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Shipping Conditions:: Blue Ice