T2040-02G
TCR, alpha, beta (T Cell Receptor, T-cell Receptor beta-1 Chain C Region, TRBC1, T-cell Receptor alpha Chain C Region, TRAC, TCRA) (FITC)
Size
£576.00
- SKU:
- T2040-02G
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Hamster
- Immunogen:
- Affinity purified mouse T cell receptor.
- Clone:
- H57-597
- Format:
- Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- The T Cell Receptor alpha/beta (TCR) is expressed on T lymphocytes of all mouse strains tested. Plate-bound or soluble antibody activates a/b TCR-bearing T cells. In vitro and in vivo, the antibody can induce immature thymocytes to undergo apoptosis. In vivo administration of this antibody has been shown to deplete a/b TCR-bearing cells to near completion and prevent graft rejection. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: use 10ul to label 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells from immunized Armenian hamsters were fused with cells of the mouse P3X63Ag.653 myeloma cell line. Recommended Negative Controls: Hamster IgG Negative Control Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice