T2035-30B-HRP

T-complex Protein 1 epsilon (T-complex Protein 1 epsilon Subunit, TCP1 epsilon, TCP1-epsilon, TCP-1 epsilon, Chaperonin Containing TCP1 epsilon, Chaperonin-containing TCP-1 epsilon, CCT epsilon, CCT-epsilon, CCTE, Chaperonin Containing TCP1 Subunit 5, Chaperonin-containing TCP-1 Subunit 5, CCT5, Chaperonin Containing TCP1 Subunit 5 epsilon, Chaperonin-containing TCP-1 Subunit 5 epsilon, KIAA0098) (HRP)

Size

£914.00

SKU:: T2035-30B-HRP
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: CCT Epsilon.
Clone:: PK/29/23/8d
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Extra Details:: The intact CCT complex is composed of eight polypeptides in a double-ring structure. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumor suppressor protein. Applications: Suitable for use in Gel Super Shift Assay, Immunoprecipitation, and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: detects a band of ~60kD in lysates of heat shocked Hela cells. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.