T2024-01

TCF3 (Transcription Factor E2-alpha, Class B Basic Helix-loop-helix Protein 21, bHLHb21, Immunoglobulin Enhancer-binding Factor E12/E47, Immunoglobulin Transcription Factor 1, Kappa-E2-binding Factor, Transcription Factor 3, TCF-3, Transcription Factor ITF-1, BHLHB21, E2A, ITF1)

Size

£748.00

SKU:: T2024-01
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: E. coli-derived recombinant protein corresponding to Gly100-Glu500 from human TCF-3.
Clone:: 10K84
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: TCF-3, also known as E2A and ITF-1, is a member of the E protein homeobox family. TCF-3 regulates checkpoints that allow B lineage commitment and B and T cell receptor gene rearrangement. Two isoforms named E12 and E47 are alternatively spliced within the DNA binding and HLH domain. The region of human TCF-3 used as the immunogen, which is identical for E12 and E47, shares 80% and 79% aa identity with mouse and rat TCF-3, respectively. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 8-25ug/ml with immersion fixed HeLa human cervical epithelial carcinoma cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.