T1017
TaqI
Size
£320.00
- SKU:
- T1017
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Extra Details:
- 5'-T ↓ C G A-3' 3'-A G C^T-5' Source: Thermus aquaticus YT-1 Supplied wtih: R1625-80: Restriction Enzyme Buffer for TaqI, 10X: Dilute to 1X for use. 1X buffer composition 10mM Tris-HCl pH 8.0, 5mM MgCl2, 100mM NaCl and 0.1mg/ml BSA. Use as 1x. R1625 - Restriction Enzyme Buffer A, 10X: Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate (pH 7.9 at 37°C), 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA Diluent Buffer: 10mM Tris-HCl pH 7.4,100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Storage Buffer: 10mM Tris-HCl pH 7.5, 300mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA and 50% glycerol. Compatible Ends: Bsp119I, Bsu15I, Hin1I, Hin6I, HpaII, MaeII, MspI, NarI, Psp1406I, Ssil, XmiI Unit Definition: One unit is defined as the amount of TaqI to digest 1ug of lambda DNA dam- in 1 hour at 65°C in 50ul of assay buffer. Thermal Inactivation: Enzyme is not inactivated by incubation at 80°C for 20min. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA dam-x 16 hours) with TaqI. Ligation/Recleavage Assay: The ligation and recleavage assay was replaced with L0 test after validating experiments showed L0 test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a factor of 100. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of TaqI for 4 hours. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice