T0725-13
TAK1-TAB1 Fusion Protein, Active
Size
£1,194.00
- SKU:
- T0725-13
- Molecular Weight:
- 42.8
- Purity:
- ~99% by SDS-PAGE and Coomassie blue staining. Purified using Ni2+/NTA-agarose.
- Storage Conditions:
- -70°C
- Supplier:
- United States Biological
- Format:
- Supplied as a liquid in 50mM Tris/HCl, pH7.5, 300mM sodium chloride, 0.1mM EGTA, 0.03% Brij 35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol.
- Extra Details:
- N-terminal His6-tagged, recombinant, human TAK1 amino acids 1-303, fused to human TAB1 amino acids 437-end, expressed by baculovirus in Sf21 insect cells. Specific Activity: ~50U/mg Unit Definition: One unit of TAK1-TAB1 fusion activity is defined as 1nmol phosphate incorporated into 2mg/ml casein per minute at 30°C with a final ATP concentration of 100uM. Applications: Suitable for use in MS Tryptic Fingerprint and Kinase Assay. Other applications not tested. Recommended Dilution: MS Tryptic Fingerprint: Confirmed identity with the translated sequence listed in the protocal. Kinase Assay: 19-48ng phosphorylated 2mg/ml casein. Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Handling Recommendations: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
- Shipping Conditions:
- Dry Ice