T0300-01
TACC3 (Transforming Acidic Coiled-coil Containing Protein 3, ERIC-1)
Size
£856.00
- SKU:
- T0300-01
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to Glu689-Ile838 of human TACC3, expressed in E. coli. Species Sequence Homology: mouse, 79%
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- Transforming acidic coiled-coil containing protein 3 (TACC3; also know as ERIC-1) is a 92-140kD member of the TACC family of transcriptional regulatory proteins. It is expressed in a variety of cell types, including Sertoli cells, endothelial cells, erythroid progenitors, and thyroid epithelium. When phosphorylated by Aurora A on Ser558, it stabilizes the mitotic spindle. It also interacts with nuclear histone acetyltransferases, promoting their positive effect on transcription. Human TACC3 is 838 amino acids in length and contains one poly-Serine region (aa155-160) and a TACC coiled-coil domain (aa637-837). There is one potential splice variant that shows a Lys substitution for aa102-462. Applications: Suitable for use in Simple Western and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using Jurkat human acute T cel leukemia cell line and K562 human chronic myelogenous leukemia cell line. Simple Western: 10ug/ml using Jurkat human acute T cell leukemia line and K562 human chronic myelogenous leukemia cell line. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-28C: IgG, H&L (HRP) Pab Dk x Gt I1904-28G: IgG, H&L (HRP) (X-Adsorbed) Pab Dk x Gt I1904-28K: IgG, H&L (HRP) Pab Rb x Gt I1904-28Q: IgG, H&L, X-Adsorbed (HRP) Pab Rb x Gt Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice