S9500-74A
Synaptopodin (SYNPO, KIAA1029)
Size
£741.00
- SKU:
- S9500-74A
- Purity:
- Hybirdoma cell culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Isolated rat kidney glomeruli.
- Clone:
- 9L671 (G1D4)
- Format:
- Supplied as a liquid, 0.09% sodium azide.
- Extra Details:
- Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7kD and pI of 9.38 (calculated from sequence data); in mouse the corresponding data are 74kD, pI 9.27. In SDS-PAGE the antigen appears as 100kD polypeptide in brain and 110kD polypeptide in kidney (the difference might be attributed to posttranslational modifications). Applications: Suitable for use in Immunofluorescence/Immunocytochemistry, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Frozen): 1:50-1:200 Immunohistochemistry (Paraffin): 1:50-1:200. Requires microwave treatment prior to incubation. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice