S9500-11X-HRP

Synuclein, alpha (BSA & Azide Free) (HRP)

Size

£1,246.00

SKU:: S9500-11X-HRP
Purity:: Purified by Protein G affinity chromatography from ascites
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human recombinant alpha-synuclein. MW: ~14kD. Cellular Localization: Neuronal cytosol and presynaptic terminals
Clone:: 3F344
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish peroxidase (HRP).
Extra Details:: Synuclein family of proteins is abundantly expressed in neuronal cytosol and presynaptic terminals. In vertebrates they are encoded by three different genes. Synucleins have been specifically implemented in three major diseases: Alzheimer’s (AD), Parkinson’s (PD) and breast cancer. In AD, a peptide derived from alpha-synuclein forms an intrinsic component of plaque amyloid. In PD, an alpha-synuclein accumulates in Lewy bodies. An allele of alpha-synuclein has been linked to many familial cases of PD. In breast cancer increased expression of gamma synuclein correlates with the disease progression. Synucleins appear to be involved in the membrane plasticity in developing song control system of songbirds. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Formalin/paraffin): Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher. Positive Control: Brain Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

Shipping Conditions:: Blue Ice