S9111-40B2
Syndecan 2 (APC)
Size
£863.00
- SKU:
- S9111-40B2
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- 4°C Do not freeze
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Glu19-Gly144 of human Syndecan 2 expressed in NSO cells.
- Clone:
- 7H79
- Format:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
- Extra Details:
- Syndecan 2 is a member of the syndecan family of Type 1 trans- membrane proteins capable of carrying heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans. The four vertebrate syndecans show conserved cytoplasmic domains and divergent extracellular portions (except for GAG attachment sites). Among the Syndecans, Syndecan 2 is most similar to Syndecan 4. Human Syndecan 2 is synthesized as 201aa core protein with an 18aa signal sequence, a 126aa extracellular domain (ECD), a 25aa transmembrane region and a 32aa cytoplasmic tail. The human ECD of Syndecan 2 contains three closely-spaced consensus Ser-Gly sequences for the attachment of HS side chains. It shares 76%, 73%, 87%, 78% and 63% aa identity with the ECD of mouse, rat, bovine, dog and chicken Syndecan 2, respectively. The cytoplasmic tail has both serine and tyrosine phosphorylation sites. Addition of 20-80 disaccharides per side chain adds considerably to the size of the 22kD core protein. Non-covalent homodimerization of Syndecan 2 is dependent on the transmembrane domain. Syndecan 2 is expressed in cells of mesenchymal origin, neuronal and epithelial cells, and is the predominant syndecan expressed during embryonic development. Expression is upregulated in several cancer cell lines. After induction in macrophages by inflammatory mediators, Syndecan 2 selectively binds FGF basic, VEGF and EGF. Syndecan 2 expressed on human primary osteoblasts binds GM-CSF and may function as a co-receptor. Activated endothelial cell Syndecan 2 specifically binds IL-8 and may participate in promoting neutrophil extravasation by forming a chemotactic IL-8 gradient. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 1x10e6 cells using COLO 205 human colorectal adenocarcinoma cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice