Syndecan 2 (MaxLight 650)

Size

£1,042.00

SKU:: S9111-40B-ML650
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to the internal region of human syndecan-2 (fibroglycan).
Format:: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
Extra Details:: MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Syndecans are heparan sulfate proteoglycans that consist of a hydrophobic membrane-spanning domain, a short cytoplasmic domain, and an extracellular domain. Syndecans contain a small core protein with distinct functional domains to which multiple glycosaminoglycan chains are linked, predominantly of the heparan sulfate variety. Syndecans are involved in cell adhesion, tissue morphogenesis and differentiation and regulation of cell responsiveness to soluble growth-regulatory compounds. These functions are attributed primarily to the glycan moieties. Syndecans also interact with growth factors such as fibroblast growth factor and epidermal growth factor. Syndecan 2 was originally cloned from embryonic fibroblasts. Syndecan 2 is expressed in epithelial-mesenchymal interfaces, and prechondrogenic and preosteogenic mesenchymal condensations, which are areas of high morphogenetic activity. Syndecan 2 is involved in the mitogenic activity and signaling of Granulocyte-Macrophage Colony-stimulating factor in osteoblasts, and has been shown to have a regulatory role in matrix assembly. Increased Syndecan 2 expression is important for colon carcinoma behavior, and Syndecan 2 regulates tumorgenic activity by regulating adhesion and proliferation in colon carcinoma cells. Applications: Suitable for use in Western Blot and FLISA. Other applications have not been tested. Recommended Dilutions: Western Blot: 1-3ug/ml FLISA: 0.1-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Shipping Conditions:: Blue Ice