S9108-19
Synaptophysin
Size
£435.00
- SKU:
- S9108-19
- Purity:
- Tissue culture supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to the C-terminal of human synaptophysin.
- Clone:
- SP11
- Format:
- Supplied as a liquid in 0.09% sodium azide.
- Extra Details:
- Synaptophysin is an ~38kD integral membrane protein involved in neurotransmitter exocytosis present on synaptic vesicles (1). This protein consists of four transmembrane domains, with its amino-and carboxyl-terminus facing the cytoplasm. Due to its transmembrane domain structure, synaptophysin has been postulated to be a gap junction-like protein as voltage-dependent channel activity is seen when synaptophysin hexamers are reconstituted into lipid membranes (2,3). Recent studies have shown synaptophysin to be a major cholesterol-binding protein in brain synaptic vesicles (4) and it has been proposed that this cholesterol-binding ability may be of key importance in the generation of synaptic vesicles at plasma membranes (5). A regional reduction of synaptophysin was found in patients with Alzheimers disease (7) and schizophrenia (6). Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: (Formalin/paraffin): 1:50 for 30 min at RT. Excellent for staining of formalin/paraffin with no special pretreatment. With pretreatment: 1:200 for 30 min. No pretreatment is necessary, however staining of formalin fixed tissues is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6 for 10-20 min followed by cooling at RT for 20 min. In combination with anti-chromogranin A and anti-NSE, antibody to synaptophysin is very useful in the identification of normal neuroendocrine cells and neuroendocrine neoplasms. Optimal dilutions to be determined by the researcher. Positive Control: Pancreas or neuroectodermal and neuroendocrine tumors Cellular Localization: Cytoplasmic Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice