S9108-16
Synaptophysin
Size
£473.00
- SKU:
- S9108-16
- Purity:
- Purified by ammonium sulfate precipitation
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synaptic vesicle-containing fractions immunoprecipitated from human brain homogenates.
- Clone:
- 4E206
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
- Extra Details:
- Synaptophysin is an ~38kD integral membrane protein involved in neurotransmitter exocytosis present on synaptic vesicles. This protein consists of four transmembrane domains, with its amino- and carboxyl-terminus facing the cytoplasm. Due to its transmembrane domain structure, synaptophysin has been postulated to be a gap junction-like protein because voltage-dependent channel activity is seen when synaptophysin hexamers are reconstituted into lipid membranes. Recent studies have shown synaptophysin to be a major cholesterol-binding protein in brain synaptic vesicles and it has been proposed that this cholesterol-binding ability may be of key importance in the generation of synaptic vesicles at plasma membranes. A regional reduction of synaptophysin was found in patients with Alzheimer’s disease and schizophrenia. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot (Colorimetric): 1:2000 Immunohistochemistry: 1:50 using human neuroblastoma tissue Optimal dilutions to be determined by researcher. Positive Controls: Mouse and rat brain tissue extract Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice